The aim of this study is to determine the IC50 of ethanol extract in India Acalypha’s herbs and roots as an antioxidant, using the DPPH method. The herbs and roots were extracted by maceration, using 96% ethanol, and then TLC method was employed for qualitative evaluation, with dichloromethane: methanol (95:5) as an eluent. The results show the presence of antioxidants, indicated by changes in colour stains from purple to yellow, after spraying with DPPH reagents. Furthermore, a UV-Vis spectrophotometer was applied in qualitative evaluation at a maximum wavelength of 515 nm, in order to obtain the IC50 value. The result confirmed that a strong antioxidant activity in the herbs and roots, based on the IC50 values of 61.80 μg/mL and 75.19 μg/mL. The antioxidant activity from the herbal ethanol extract Anting-anting (top parts) and roots (bottom parts) (Acalypha indica L.) was moderate, with IC50 values of 61.80 μg / mL, and 75.19 μg / mL, respectively. Meanwhile, the Quercetin used as a comparison standard has an IC50 of <10 μg/mL, or 2,438 μg/mL. Therefore, it is important to encourage the consumption of S. Jambos, in order to promote community health benefits.
Volume 12 | Issue 5
Pages: 465-468
DOI: 10.5373/JARDCS/V12I5/20201961